Welcome to the Biotage Sample Preparation Blog

      Stephanie J. Marin, Ph.D.


      Recent Posts

      How to choose the best elution solvent for SLE?

      September 24, 2021 at 2:18 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, solvent polarity, elution

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      Choosing the SLE correct elution solvent based on analyte properties produces the cleanest extracts with the highest recoveries.

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      How do I pretreat my samples for Supported Liquid Extraction

      July 14, 2021 at 6:09 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, pretreatment

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      Correct pretreatment of samples prior to supported liquid extraction (SLE) produces better partitioning and cleaner extracts. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      Why should I underload SLE+ plate or cartridge?

      July 7, 2021 at 7:00 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction

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      Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      When should I choose SPE instead of SLE?

      October 9, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Solid-phase extraction

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      When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques. 

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      Protein precipitation vs. traditional protein crash: what's best?

      October 7, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in Sample preparation, Method development, Protein precipitation

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      Most clinical chemists have developed a blood, serum or plasma assay using a protein crash because it is inexpensive and generally removes proteins that interfere with detection or the analysis in some way. But is this always true?

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      How do I Develop a Sample Prep Method?

      October 2, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in LC-MS, Sample preparation

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      This is a question that I am often asked by very smart people that just haven’t had time to learn the process. An optimized sample preparation method is critical for an accurate, specific, robust clinical LC-MS/MS assay. A good sample prep method can improve accuracy and precision, provide longer LC column lifetime, and keep your LC-MS/MS system clean.

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      What is Crosstalk and how do I Prevent it from Contaminating my Samples?

      September 18, 2020 at 1:47 PM / by Stephanie J. Marin, Ph.D. posted in amphetamines, contamination, false positive result

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      Evaporative crosstalk is contamination that occurs during evaporation of the elution solvent after SLE or SPE extraction. Volatile analytes evaporate, then redistribute in adjacent wells in a 96-well plate. This can generate false positive results and require re-extraction of patient specimens. Addition of HCl in methanol (MeOH) to produce the salt of volatile compounds like sympathomimetic amines is used to control crosstalk. The type of evaporator, conditions and solvents used can all contribute to crosstalk. 

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      Methods for a drug class or one large panel - what's the difference

      August 23, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in LC-MS, GC-MS, Extraction method optimization, Sample preparation, Method development

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      Most clinical and forensic labs have a used a traditional approach for drug testing called screen with reflex to confirmation. This involves analyzing samples using an immunoassay technique that identifies a drug class. Positive immunoassay results are then analyzed by a second more specific method, like GC-MS or LC-MS/MS, to identify and quantitate specific drug analytes.  

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      When should I choose weak ion exchange SPE phases?

      July 8, 2020 at 3:17 PM / by Stephanie J. Marin, Ph.D. posted in SPE solid phase extraction, Extraction method optimization, Sample preparation

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      What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SPE phases - EVOLUTE EXPRESS WCX and EVOLUTE EXPRESS WAX - and how to optimize your sample preparation of your analytes of interest.

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      When should I choose a mixed-mode SPE?

      July 8, 2020 at 3:15 PM / by Stephanie J. Marin, Ph.D. posted in SPE solid phase extraction, Evolute Express AX, Evolute Express CX

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      Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interaction during SPE is useful when a large number of analytes with different properties are of interest.

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