Welcome to the Biotage Sample Preparation Blog

      Jillian Neifeld, MS


      Recent Posts

      How do I come up with a starting point for my SPE method?

      September 22, 2020 at 4:15 PM / by Jillian Neifeld, MS posted in SPE, Solid-phase extraction

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      You've just decided to develop a solid phase extraction (SPE) method for a new assay in your lab. However, there are so many decisions to be made! Which company to choose? Which product to use? Polymer based sorbent or silica based? Cation or anion exchange? While this seems incredibly difficult, it doesn't have to be that hard!

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      Synthetic Cannabinoids: how to extract them from whole blood?

      August 26, 2020 at 2:41 PM / by Jillian Neifeld, MS posted in SLE Supported Liquid Extraction, Forensic, Clinical, Whole Blood, Cannabinoids

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      Synthetic cannabinoids are some of the most widely abused drugs in the world. They are structurally similar and have similar effects to marijuana but are frequently referred to as “legal highs”. Chemists are constantly changing the structures of these compounds to keep them in a legal state and governments can’t keep up with adding them to scheduled lists. So, how do we analyze for these compounds? How can they be easily extracted from whole blood?

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      Why should I filter my samples instead of doing dilute-and-shoot?

      August 26, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in Extraction method optimization, Sample preparation, Method development

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      Let's face it, the whole solid phase extraction method development process can be time consuming and expensive. So Dilute-and-Shoot methods end up being the choice in some laboratories. The problem is that these methods are dirty and lead to a lot of other issues. In this blog post, I'll be discussing filtration methods and how those are a superior option to Dilute-and-Shoot.

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      How to extract and analyze for ever-present contaminants of interest?

      August 6, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SLE Supported Liquid Extraction, Sample preparation, Method development

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      It happens to all of us. We're getting a new method developed and validated and then it comes time to run our negative urines. And everything comes up as positive! There are peaks for our analytes of interest in every urine that we run! How is that possible?

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      Vacuum or positive pressure for processing samples: what's best?

      July 29, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SPE, Extraction method optimization, Sample preparation, Solid-phase extraction

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      Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.

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      Determining the Optimal Sample Size for your Sorbent Bed Mass

      July 26, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in Extraction method optimization, Sample preparation, Evolute Express CX, Sorbent, Method development

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      In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass.  We have developed an adequate solid phase extraction method for our laboratory. However, we're starting to notice some oddities when we extract and analyze our samples. Could we be losing compounds from our sample?

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      How does my sample prep change between GC/MS and LC/MS/MS?

      July 23, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in LC-MS, GC-MS, Sample preparation

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      LC/MS/MS seems to be the gold standard in sample analysis nowadays. However, some labs are still analyzing samples using GC/MS. The majority of published peer-reviewed articles are for LC/MS/MS, so those working with GC/MS instrumentation may not know how to change the sample preparation to better fit GC/MS.

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      In-well hydrolysis plates: What are they and do they really work?

      June 16, 2020 at 2:23 PM / by Jillian Neifeld, MS posted in Hydrolysis plates

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      One of the key features of the so called "in-well hydrolysis" is that it can be a time saver for high throughput labs, which makes it look very appealing. In this blog post, I will be discussing some of the advantages of using an in-well hydrolysis plate and some of the results that I’ve gotten when compared to an out-of-well hydrolysis plate.

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      Urine hydrolysis: how did I choose which enzyme to use?

      June 16, 2020 at 2:15 PM / by Jillian Neifeld, MS

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      Urine hydrolysis is a part of sample preparation that we do to eliminate glucuronides before urine analysis. So first, what are glucuronides? And why do we want to get rid of them? In this post, I’ll tell you about my experience with urine hydrolysis and my success (or lack of success) when using different enzymes.

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      Synthetic Opioids: What are they and how do we analyze them?

      May 13, 2020 at 4:24 PM / by Jillian Neifeld, MS posted in Automation, SLE Supported Liquid Extraction, Drug Metabolites, Extrahera

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      Novel Psychoactive Substances (NPSs) are drugs that have been made to have similar effects to common drugs of abuse. For example, synthetic opioids like fentanyl were created to make the user think they were taking heroin. However, these synthetic compounds have potencies that are sometimes much higher than their similar, more common drug of abuse.

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