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      Dan Menasco


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      What are the first steps to building a proper method?

      September 4, 2020 at 8:40 AM / by Dan Menasco posted in Chromatography Fundamentals, Detector

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      Before we get into our extractions, we need to set up our LC/MS to detect our analytes of interest. The LC will provide the chromatography - or separation of our analytes - and the MS will support detection. So which one do we evaluate first? Well, if you can’t “see” the analytes, what good is perfecting their chromatography? So, first, let’s get the MS focused, or tuned, for optimum analyte detection.

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      Trying to develop a new method? How do I do a literature search?

      September 4, 2020 at 8:34 AM / by Dan Menasco posted in Sample preparation, Method development

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      When trying to develop a new method for the detection of a drug panel, you need to refer to reliable sources to learn all you need to know regarding your analytes. For this post, we’ll go in detail through the search process with analytes like naloxone, buprenorphine, norfentanyl and methadone using urine as our matrix of focus.

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      Need good sources of reference for sample prep method development?

      September 4, 2020 at 8:33 AM / by Dan Menasco posted in Sample preparation, Method development

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      The sole purpose for method development is to construct a robust and analytically sound method that will not just pass the barriers of validation, but provide physicians and patients with sound and reliable results. But where to start?

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      How to Monitor and Prevent Sample Carryover during Method Development

      July 19, 2020 at 8:00 AM / by Dan Menasco posted in Troubleshooting and Optimization, Extraction method optimization, Sample preparation

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      When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, the last thing we want to see is some random hiccup in our workflow. But a whole validation without a hiccup is just wishful thinking, right.

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      How to determine recovery and matrix effects for your analytical assay

      June 30, 2020 at 10:50 AM / by Dan Menasco posted in SLE Supported Liquid Extraction, Extraction method optimization

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      For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay really? How do we measure reliability of our assay?

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