Why is pH adjustment important for sample prep methods?

June 30, 2020 at 2:08 PM / by Stephanie J. Marin, Ph.D. posted in SPE, Extraction method optimization, Sample preparation

How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what conditions, so you can use this property to develop better sample prep methods.

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How to determine recovery and matrix effects for your analytical assay

June 30, 2020 at 10:50 AM / by Dan Menasco posted in SLE Supported Liquid Extraction, Extraction method optimization

For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay really? How do we measure reliability of our assay?

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What’s the Best Way to do Supported Liquid Extraction?

June 23, 2020 at 4:09 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation

Supported Liquid Extraction (SLE) is a sample preparation technique that has been in use for over ten years now, but many analytical chemists don’t know about, or understand the best way to do an SLE extraction. In this post, I’m going to talk about how SLE works and the proper way to do an SLE extraction for sample clean up.

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How to develop a Supported Liquid Extraction method in 16 easy steps

June 23, 2020 at 4:00 PM / by Bruce Kempf posted in SLE Supported Liquid Extraction, Extraction method optimization

Taking the time to develop a Supported Liquid Extraction (SLE) method using these 16 easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting the best recoveries possible.

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In-well hydrolysis plates: What are they and do they really work?

June 16, 2020 at 2:23 PM / by Jillian Neifeld, MS posted in Hydrolysis plates

One of the key features of the so called "in-well hydrolysis" is that it can be a time saver for high throughput labs, which makes it look very appealing. In this blog post, I will be discussing some of the advantages of using an in-well hydrolysis plate and some of the results that I’ve gotten when compared to an out-of-well hydrolysis plate.

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Urine hydrolysis: how did I choose which enzyme to use?

June 16, 2020 at 2:15 PM / by Jillian Neifeld, MS

Urine hydrolysis is a part of sample preparation that we do to eliminate glucuronides before urine analysis. So first, what are glucuronides? And why do we want to get rid of them? In this post, I’ll tell you about my experience with urine hydrolysis and my success (or lack of success) when using different enzymes.

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What is the Role of LogP in Sample Prep Methods?

June 10, 2020 at 3:30 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE

Understanding the chemical properties of the compounds in your sample matrix, both the ones you want to detect and the ones you want to eliminate, is necessary for successful method development. Is the molecule acidic or basic? What functional groups are present and can they be ionized? Is the molecule hydrophilic or hydrophobic? In this post, we discuss logP, and its role in sample preparation.

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How to choose between SPE and SLE?

June 10, 2020 at 3:30 PM / by Simonetta Tumbiolo posted in SLE Supported Liquid Extraction, SPE, Extraction method optimization

Confused about how to choose the right sample preparation technique for your compound? Don't know if Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE) would be best for your sample preparation? We are here to help you select the most efficient extraction method for your sample, step by step, in the easiest possible way.

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