How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what conditions, so you can use this property to develop better sample prep methods.
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For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay really? How do we measure reliability of our assay?
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Supported Liquid Extraction (SLE) is a sample preparation technique that has been in use for over ten years now, but many analytical chemists don’t know about, or understand the best way to do an SLE extraction. In this post, I’m going to talk about how SLE works and the proper way to do an SLE extraction for sample clean up.
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Taking the time to develop a Supported Liquid Extraction (SLE) method using easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting the best recoveries possible.
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Urine hydrolysis is a part of sample preparation that we do to eliminate glucuronides before urine analysis. So first, what are glucuronides? And why do we want to get rid of them? In this post, I’ll tell you about my experience with urine hydrolysis and my success (or lack of success) when using different enzymes.
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Understanding the chemical properties of the compounds in your sample matrix, both the ones you want to detect and the ones you want to eliminate, is necessary for successful method development. Is the molecule acidic or basic? What functional groups are present and can they be ionized? Is the molecule hydrophilic or hydrophobic? In this post, we discuss logP, and its role in sample preparation.
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Confused about how to choose the right sample preparation technique for your compound? Don't know if Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE) would be best for your sample preparation? We are here to help you select the most efficient extraction method for your sample, step by step, in the easiest possible way.
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