Smarter Sample Prep for Vitamin Testing


A Complete Guide to Bioanalytical Sample Preparation

You analyze a lot of biological samples. You deliver accurate, reliable data, consistently. You use sophisticated, high-end analytical techniques such as LC-MS/MS or GC-MS. And the pressure is always there to do it faster, with greater sensitivity and, if possible, at lower cost. Sample preparation can be critical for success.

Learn more here on how to choose the right approach for your samples.

Bioanalytical Sample Prep

Application Note 857

Trending Application Notes

Ultra-Sensitive Method for Determining Vitamin D2 and D3 Metabolites in Serum

A new application from Biotage Labs describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. Read More

Trending Application Notes

Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN

Vitamin B7 (Biotin) is a water-soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate. Read More

Application Note 880

Application Note 842

Trending Application Notes

Extraction of 25-hydroxy Vitamin D from Serum Using
ISOLUTE® PLD+ Prior to LC-MS/MS Analysis

This application note describes the extraction of vitamin D metabolites from serum using ISOLUTE® PLD+ protein and phospholipid removal plates. This simple clean up technique provides an alternative approach to supported liquid extraction, using water miscible solvents (acetonitrile) for the extraction. Read More

Highlighted Products

ISOLUTE® SLE+ Supported Liquid Extraction 

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns are designed to provide stress free extraction of analytes from biological fluids, with high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple. Read More



Highlighted Products


EVOLUTE® EXPRESS Sample Preparation products provide robust, reliable SPE, giving ultra-pure extracts and high analyte recoveries. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE sorbents provide a highly effective solution to the problems of ion suppression and matrix effects. Eliminate conditioning and equilibration with EVOLUTE EXPRESS load-wash-elute SPE. Read More

Clinical Applications Compendium

Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.




Clinical Applications Compendium