You analyze a lot of biological samples. You deliver accurate, reliable data, consistently. You use sophisticated, high-end analytical techniques such as LC-MS/MS or GC-MS. And the pressure is always there to do it faster, with greater sensitivity and, if possible, at lower cost. Sample preparation can be critical for success.
Learn more here on how to choose the right approach for your samples.
A new application from Biotage Labs describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. Read More
Vitamin B7 (Biotin) is a water-soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate. Read More
This application note describes the extraction of vitamin D metabolites from serum using ISOLUTE® PLD+ protein and phospholipid removal plates. This simple clean up technique provides an alternative approach to supported liquid extraction, using water miscible solvents (acetonitrile) for the extraction. Read More
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns are designed to provide stress free extraction of analytes from biological fluids, with high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple. Read More
EVOLUTE® EXPRESS Sample Preparation products provide robust, reliable SPE, giving ultra-pure extracts and high analyte recoveries. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE sorbents provide a highly effective solution to the problems of ion suppression and matrix effects. Eliminate conditioning and equilibration with EVOLUTE EXPRESS load-wash-elute SPE. Read More
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.