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      How to Reduce Sample Volumes without Sacrificing Analytical Detection Limits

      October 21, 2021 at 2:00 PM / by Deanna Bissonnette posted in biotage horizon 5000, oil and grease, wastewater, epa method 1664b, Biotage Horizon 3100, hexane extractable materials, EPA, reduced volume


      In the world of environmental testing, many labs encounter extremely dirty samples. For example, EPA Method 1664B is the protocol for extracting oil and grease from wastewater samples. More often than not, these types of samples are really dirty, requiring loads of pre-filters, glass wool, and filter aid in order to get the entire liter of water through the disk. Well, there is! We know that laboratories are always looking for ways to speed up the extraction process and reducing the volume of the sample to be extracted is one of the best places to start.

      For our “sample reduction study” we chose to follow along with EPA Method 1664B because this is the protocol for wastewater samples. They are usually the most difficult samples to extract an entire liter of, due to the nature of the different varieties of sample matrices from discharge locations. Per EPA Method 1664B section, you have the ability to reduce your sample volumes from one liter to something smaller. The method specifically outlines the use of 100 mL sample volumes. The idea of reducing your sample volumes to 100 mL is very appealing, especially for samples that have a hard time filtering through even with all the filter aid, but there were some issues with accuracy on the gravimetric side. When you are reducing your sample volumes 10-fold you need to make sure that you can compensate for that reduction on the gravimetric side with a balance capable of measuring 10x more accurately. View the table below, to see what I mean:

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      Why It’s Easier to Succeed With Wastewater Extractions

      December 10, 2020 at 2:38 PM / by Maura Rury posted in SPE solid phase extraction, application, wastewater, HEM, e-book, hexane extractable materials


      There’s nothing more satisfying than successfully extracting a really challenging sample. Solid phase extraction (SPE) is a powerful technique for extracting semi-volatile organic compounds and hexane-extractable materials (HEMs). When the chemistry is tailored to meet the requirements of the application, literally hundreds of compounds can be extracted with a single pass of solution through an SPE disk.

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