Microelution plate processing: What pressure is best?
March 16, 2021 at 3:33 PM / by Charlotte Hayes posted in Troubleshooting and Optimization, Extraction method optimization, Microelution, SPE, positive pressure processing
Troubleshooting Loss of Signal: Where did my peaks go?
October 25, 2020 at 7:45 AM / by Dan Menasco posted in Troubleshooting and Optimization, Sample preparation
Let’s take a look at one of the more irritating issues in LC/MS method development: signal loss. I’m not talking about signal attenuation, which can be much more convoluted to diagnose; but rather a complete loss of signal – nothing, not even a blip on your TIC. So where do we begin?
There and Square Again: A Frit Coverage Tale
August 20, 2020 at 8:00 AM / by Jeremy Smith posted in Troubleshooting and Optimization, Loading Techniques, SLE Supported Liquid Extraction, Sample preparation
Have you ever encountered problems loading your samples onto an SPE or SLE plate? Aside from sample viscosity or cartridge blockages, a good troubleshooting step to start with is ensuring that you are achieving complete frit coverage on the load.
How to Monitor and Prevent Sample Carryover during Method Development
July 19, 2020 at 8:00 AM / by Dan Menasco posted in Troubleshooting and Optimization, Extraction method optimization, Sample preparation