Welcome to the Biotage Sample Preparation Blog

      When should I choose SPE instead of SLE?

      October 9, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Solid-phase extraction

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      When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques. 

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      How do I come up with a starting point for my SPE method?

      September 22, 2020 at 4:15 PM / by Jillian Neifeld, MS posted in SPE solid phase extraction, Solid-phase extraction

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      You've just decided to develop a solid phase extraction (SPE) method for a new assay in your lab. However, there are so many decisions to be made! Which company to choose? Which product to use? Polymer based sorbent or silica based? Cation or anion exchange? While this seems incredibly difficult, it doesn't have to be that hard!

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      Techniques for disrupting protein binding in sample preparation

      August 2, 2020 at 8:00 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Sample preparation, Solid-phase extraction, Method development

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      If you are frustrated with low recoveries caused by protein binding during Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE), this blog post will give you a few tips. Proper sample pre-treatment is the key to solving this issue.

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      Vacuum or positive pressure for processing samples: what's best?

      July 29, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SPE solid phase extraction, Extraction method optimization, Sample preparation, Solid-phase extraction

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      Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.

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      Choosing the best Ion Exchange Mode for Solid Phase Extraction

      July 16, 2020 at 8:00 AM / by Bruce Kempf posted in SPE solid phase extraction, Solid-phase extraction, ION exchange, Evolute Express

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      There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple guidelines on making the best choice. You will see that ion exchange is all about acids and bases.

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      When should I choose SLE or SPE for sample cleanup?

      July 13, 2020 at 9:21 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE solid phase extraction

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      When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s right for you depends on your application and the characteristics of your analytes. This post outlines some of the advantages, disadvantages and limitations of each technique.

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      When should I choose weak ion exchange SPE phases?

      July 8, 2020 at 3:17 PM / by Stephanie J. Marin, Ph.D. posted in SPE solid phase extraction, Extraction method optimization, Sample preparation

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      What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SPE phases - EVOLUTE EXPRESS WCX and EVOLUTE EXPRESS WAX - and how to optimize your sample preparation of your analytes of interest.

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      When should I choose a mixed-mode SPE?

      July 8, 2020 at 3:15 PM / by Stephanie J. Marin, Ph.D. posted in SPE solid phase extraction, Evolute Express AX, Evolute Express CX

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      Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interaction during SPE is useful when a large number of analytes with different properties are of interest.

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      Why is pH adjustment important for sample prep methods?

      June 30, 2020 at 2:08 PM / by Stephanie J. Marin, Ph.D. posted in SPE solid phase extraction, Extraction method optimization, Sample preparation

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      How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what conditions, so you can use this property to develop better sample prep methods.

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      What is the Role of LogP in Sample Prep Methods?

      June 10, 2020 at 3:30 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE solid phase extraction

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      Understanding the chemical properties of the compounds in your sample matrix, both the ones you want to detect and the ones you want to eliminate, is necessary for successful method development. Is the molecule acidic or basic? What functional groups are present and can they be ionized? Is the molecule hydrophilic or hydrophobic? In this post, we discuss logP, and its role in sample preparation.

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