Welcome to the Biotage Sample Preparation Blog

      How to achieve faster sample prep, cleaner extracts, and instant sensitivity gains

      October 15, 2020 at 9:06 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to meet goals for detection limits. What's best between conventional solid phase extraction (SPE) and microelution SPE?

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      The revolution of microelution in solid phase extraction

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be an effective tool when bioanalysis demands higher sensitivity and reproducibility with decreasing sample volumes? In theory, SPE can be used for targeted extractions with ultra-high concentrations. As we will see, putting this into practice requires miniaturization of SPE extraction using sorbent beds with optimal formats that minimize elution volume to maximize detection and quantitation power.

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      How to meet demands for detection limits with the right sample prep

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volumes. Achieving this performance involves pushing the limits of detection and quantitation with high levels of sensitivity and reproducibility that are only possible with high quality samples.

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      When should I choose SPE instead of SLE?

      October 9, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE, Solid-phase extraction

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      When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques. 

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      How do I come up with a starting point for my SPE method?

      September 22, 2020 at 4:15 PM / by Jillian Neifeld, MS posted in SPE, Solid-phase extraction

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      You've just decided to develop a solid phase extraction (SPE) method for a new assay in your lab. However, there are so many decisions to be made! Which company to choose? Which product to use? Polymer based sorbent or silica based? Cation or anion exchange? While this seems incredibly difficult, it doesn't have to be that hard!

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      Techniques for disrupting protein binding in sample preparation

      August 2, 2020 at 8:00 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE, Sample preparation, Solid-phase extraction, Method development

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      If you are frustrated with low recoveries caused by protein binding during Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE), this blog post will give you a few tips. Proper sample pre-treatment is the key to solving this issue.

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      Vacuum or positive pressure for processing samples: what's best?

      July 29, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SPE, Extraction method optimization, Sample preparation, Solid-phase extraction

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      Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.

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      Choosing the best Ion Exchange Mode for Solid Phase Extraction

      July 16, 2020 at 8:00 AM / by Bruce Kempf posted in SPE, Solid-phase extraction, ION exchange, Evolute Express

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      There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple guidelines on making the best choice. You will see that ion exchange is all about acids and bases.

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