Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be an effective tool when bioanalysis demands higher sensitivity and reproducibility with decreasing sample volumes? In theory, SPE can be used for targeted extractions with ultra-high concentrations. As we will see, putting this into practice requires miniaturization of SPE extraction using sorbent beds with optimal formats that minimize elution volume to maximize detection and quantitation power.
Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volumes. Achieving this performance involves pushing the limits of detection and quantitation with high levels of sensitivity and reproducibility that are only possible with high quality samples.
When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques.
You've just decided to develop a solid phase extraction (SPE) method for a new assay in your lab. However, there are so many decisions to be made! Which company to choose? Which product to use? Polymer based sorbent or silica based? Cation or anion exchange? While this seems incredibly difficult, it doesn't have to be that hard!
If you are frustrated with low recoveries caused by protein binding during Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE), this blog post will give you a few tips. Proper sample pre-treatment is the key to solving this issue.
Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.