Welcome to the Biotage Sample Preparation Blog

      Techniques for disrupting protein binding in sample preparation

      August 2, 2020 at 8:00 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Sample preparation, Solid-phase extraction, Method development

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      If you are frustrated with low recoveries caused by protein binding during Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE), this blog post will give you a few tips. Proper sample pre-treatment is the key to solving this issue.

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      When should I choose SLE or SPE for sample cleanup?

      July 13, 2020 at 9:21 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE solid phase extraction

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      When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s right for you depends on your application and the characteristics of your analytes. This post outlines some of the advantages, disadvantages and limitations of each technique.

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      How to determine recovery and matrix effects for your analytical assay

      June 30, 2020 at 10:50 AM / by Dan Menasco posted in SLE Supported Liquid Extraction, Extraction method optimization

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      For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay really? How do we measure reliability of our assay?

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      What’s the Best Way to do Supported Liquid Extraction?

      June 23, 2020 at 4:09 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation

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      Supported Liquid Extraction (SLE) is a sample preparation technique that has been in use for over ten years now, but many analytical chemists don’t know about, or understand the best way to do an SLE extraction.  In this post, I’m going to talk about how SLE works and the proper way to do an SLE extraction for sample clean up.

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      Easy protocol to develop your Supported Liquid Extraction method

      June 23, 2020 at 4:00 PM / by Bruce Kempf posted in SLE Supported Liquid Extraction, Extraction method optimization

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      Taking the time to develop a Supported Liquid Extraction (SLE) method using  easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting the best recoveries possible.

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      What is the Role of LogP in Sample Prep Methods?

      June 10, 2020 at 3:30 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE solid phase extraction

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      Understanding the chemical properties of the compounds in your sample matrix, both the ones you want to detect and the ones you want to eliminate, is necessary for successful method development. Is the molecule acidic or basic? What functional groups are present and can they be ionized? Is the molecule hydrophilic or hydrophobic? In this post, we discuss logP, and its role in sample preparation.

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      How to choose between SPE and SLE?

      June 10, 2020 at 3:30 PM / by Simonetta Tumbiolo posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Extraction method optimization

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      Confused about how to choose the right sample preparation technique for your compound? Don't know if Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE) would be best for your sample preparation? We are here to help you select the most efficient extraction method for your sample, step by step, in the easiest possible way.

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      Synthetic Opioids: What are they and how do we analyze them?

      May 13, 2020 at 4:24 PM / by Jillian Neifeld, MS posted in Automation, SLE Supported Liquid Extraction, Drug Metabolites, Extrahera

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      Novel Psychoactive Substances (NPSs) are drugs that have been made to have similar effects to common drugs of abuse. For example, synthetic opioids like fentanyl were created to make the user think they were taking heroin. However, these synthetic compounds have potencies that are sometimes much higher than their similar, more common drug of abuse.

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      Automating Sample Prep Boosts Analytical Lab Efficiency

      February 18, 2020 at 8:20 AM / by Nigel Tooke posted in Automation, SLE Supported Liquid Extraction, Extrahera

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      Automated sample prep helps your lab to run more smoothly. Sample prep is often seen as a painful process that is time-consuming, laborious, complex, error-prone, and costly. But efficient sample prep is a critical step that is required to supply high performance analytical equipment with the best possible input to routinely generate high quality data output. 

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      High Quality Sample Prep is Critical in Analytical Labs

      February 18, 2020 at 8:19 AM / by Nigel Tooke posted in Automation, SLE Supported Liquid Extraction, SPE solid phase extraction, Quality of Biotage Products, LC-MS, GC-MS

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      Analytical laboratories using techniques such as LC-MS/MS or GC-MS are continuously searching for ways to increase throughput, reduce turnaround times and lower costs. Sample prep is often an underestimated component in this process, but is a critical step that removes matrix interferences, concentrates samples, and also protects expensive analytical instrumentation. 

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