Welcome to the Biotage Sample Preparation Blog

      How to choose the best elution solvent for SLE?

      September 24, 2021 at 2:18 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, solvent polarity, elution

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      Choosing the SLE correct elution solvent based on analyte properties produces the cleanest extracts with the highest recoveries.

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      How do I pretreat my samples for Supported Liquid Extraction

      July 14, 2021 at 6:09 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, pretreatment

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      Correct pretreatment of samples prior to supported liquid extraction (SLE) produces better partitioning and cleaner extracts. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      Why should I underload SLE+ plate or cartridge?

      July 7, 2021 at 7:00 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction

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      Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      Why just measure Vitamin D when you can monitor Vitamins A, E and K as well?

      November 11, 2020 at 2:58 PM / by Alan Edgington posted in SLE Supported Liquid Extraction, Sample preparation, Vitamins

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      A new method has been developed for the combined analysis of Vitamin A, Vitamin D, Vitamin E and Vitamin K from serum using LC-MS/MS. The method uses supported liquid extraction (SLE) with a novel sample load which could also potentially be employed for many other highly non-polar analytes that have limited water solubility and are highly protein bound within the body.

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      Dilute & shoot ... yourself in the foot

      October 21, 2020 at 8:30 AM / by Simonetta Tumbiolo posted in SLE Supported Liquid Extraction, Sample preparation, Method development, Clinical

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      Urine samples are composed of many interferents which can cause ion-suppression or ion-enhancement when analyzing by mass spec. Build up of these matrix components over multiple sample injections can lead to loss of signal in the mass spec and even unexpected changes in your chromatographic separation. We are going to discuss why not performing adequate sample prep can be detrimental for your analyses.

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      When should I choose SPE instead of SLE?

      October 9, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE solid phase extraction, Solid-phase extraction

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      When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques. 

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      Synthetic Cannabinoids: how to extract them from whole blood?

      August 26, 2020 at 2:41 PM / by Jillian Neifeld, MS posted in SLE Supported Liquid Extraction, Forensic, Clinical, Whole Blood, Cannabinoids

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      Synthetic cannabinoids are some of the most widely abused drugs in the world. They are structurally similar and have similar effects to marijuana but are frequently referred to as “legal highs”. Chemists are constantly changing the structures of these compounds to keep them in a legal state and governments can’t keep up with adding them to scheduled lists. So, how do we analyze for these compounds? How can they be easily extracted from whole blood?

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      There and Square Again: A Frit Coverage Tale

      August 20, 2020 at 8:00 AM / by Jeremy Smith posted in Troubleshooting and Optimization, Loading Techniques, SLE Supported Liquid Extraction, Sample preparation

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      Have you ever encountered problems loading your samples onto an SPE or SLE plate?  Aside from sample viscosity or cartridge blockages, a good troubleshooting step to start with is ensuring that you are achieving complete frit coverage on the load.

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      Extracting both acidic and basic analytes in a single SLE procedure

      August 9, 2020 at 8:00 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction

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      Often the question arises asking how can I extract both acids and bases with the same Supported Liquid Extraction (SLE) procedure. Is this possible? And how?

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      How to extract and analyze for ever-present contaminants of interest?

      August 6, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SLE Supported Liquid Extraction, Sample preparation, Method development

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      It happens to all of us. We're getting a new method developed and validated and then it comes time to run our negative urines. And everything comes up as positive! There are peaks for our analytes of interest in every urine that we run! How is that possible?

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