Welcome to the Biotage Sample Preparation Blog

      Microelution plate processing: What pressure is best?

      March 16, 2021 at 3:33 PM / by Charlotte Hayes posted in Troubleshooting and Optimization, Extraction method optimization, Microelution, SPE, positive pressure processing

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      Microelution SPE is a great alternative to conventional SPE methods, enabling the use of lower wash and elution volumes, while maintaining sufficient load volumes, providing concentrated samples, perfect for highly sensitive analysis. Achieving the best, most consistent results means using the right processing conditions, and because all microelution SPE plates are not the same, you might need to adjust your processing conditions when using these plates.

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      How to achieve faster sample prep, cleaner extracts, and instant sensitivity gains

      October 15, 2020 at 9:06 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to meet goals for detection limits. What's best between conventional solid phase extraction (SPE) and microelution SPE?

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      The revolution of microelution in solid phase extraction

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be an effective tool when bioanalysis demands higher sensitivity and reproducibility with decreasing sample volumes? In theory, SPE can be used for targeted extractions with ultra-high concentrations. As we will see, putting this into practice requires miniaturization of SPE extraction using sorbent beds with optimal formats that minimize elution volume to maximize detection and quantitation power.

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      How to meet demands for detection limits with the right sample prep

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volumes. Achieving this performance involves pushing the limits of detection and quantitation with high levels of sensitivity and reproducibility that are only possible with high quality samples.

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