Microelution plate processing: What pressure is best?
March 16, 2021 at 3:33 PM / by Charlotte Hayes posted in Troubleshooting and Optimization, Extraction method optimization, Microelution, SPE, positive pressure processing
How to achieve faster sample prep, cleaner extracts, and instant sensitivity gains
October 15, 2020 at 9:06 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution
A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to meet goals for detection limits. What's best between conventional solid phase extraction (SPE) and microelution SPE?
The revolution of microelution in solid phase extraction
October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution
Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be an effective tool when bioanalysis demands higher sensitivity and reproducibility with decreasing sample volumes? In theory, SPE can be used for targeted extractions with ultra-high concentrations. As we will see, putting this into practice requires miniaturization of SPE extraction using sorbent beds with optimal formats that minimize elution volume to maximize detection and quantitation power.
How to meet demands for detection limits with the right sample prep
October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution