Welcome to the Biotage Sample Preparation Blog

      Why should I filter my samples instead of doing dilute-and-shoot?

      August 26, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in Extraction method optimization, Sample preparation, Method development

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      Let's face it, the whole solid phase extraction method development process can be time consuming and expensive. So Dilute-and-Shoot methods end up being the choice in some laboratories. The problem is that these methods are dirty and lead to a lot of other issues. In this blog post, I'll be discussing filtration methods and how those are a superior option to Dilute-and-Shoot.

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      Methods for a drug class or one large panel - what's the difference

      August 23, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in LC-MS, GC-MS, Extraction method optimization, Sample preparation, Method development

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      Most clinical and forensic labs have a used a traditional approach for drug testing called screen with reflex to confirmation. This involves analyzing samples using an immunoassay technique that identifies a drug class. Positive immunoassay results are then analyzed by a second more specific method, like GC-MS or LC-MS/MS, to identify and quantitate specific drug analytes.  

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      Vacuum or positive pressure for processing samples: what's best?

      July 29, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SPE, Extraction method optimization, Sample preparation, Solid-phase extraction

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      Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.

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      Determining the Optimal Sample Size for your Sorbent Bed Mass

      July 26, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in Extraction method optimization, Sample preparation, Evolute Express CX, Sorbent, Method development

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      In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass.  We have developed an adequate solid phase extraction method for our laboratory. However, we're starting to notice some oddities when we extract and analyze our samples. Could we be losing compounds from our sample?

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      How to Monitor and Prevent Sample Carryover during Method Development

      July 19, 2020 at 8:00 AM / by Dan Menasco posted in Troubleshooting and Optimization, Extraction method optimization, Sample preparation

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      When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, the last thing we want to see is some random hiccup in our workflow. But a whole validation without a hiccup is just wishful thinking, right.

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      When should I choose weak ion exchange SPE phases?

      July 8, 2020 at 3:17 PM / by Stephanie J. Marin, Ph.D. posted in SPE, Extraction method optimization, Sample preparation

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      What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SPE phases - EVOLUTE EXPRESS WCX and EVOLUTE EXPRESS WAX - and how to optimize your sample preparation of your analytes of interest.

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      Why is pH adjustment important for sample prep methods?

      June 30, 2020 at 2:08 PM / by Stephanie J. Marin, Ph.D. posted in SPE, Extraction method optimization, Sample preparation

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      How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what conditions, so you can use this property to develop better sample prep methods.

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      How to determine recovery and matrix effects for your analytical assay

      June 30, 2020 at 10:50 AM / by Dan Menasco posted in SLE Supported Liquid Extraction, Extraction method optimization

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      For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay really? How do we measure reliability of our assay?

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      How to develop a Supported Liquid Extraction method in 16 easy steps

      June 23, 2020 at 4:00 PM / by Bruce Kempf posted in SLE Supported Liquid Extraction, Extraction method optimization

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      Taking the time to develop a Supported Liquid Extraction (SLE) method using these 16 easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting the best recoveries possible.

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      How to choose between SPE and SLE?

      June 10, 2020 at 3:30 PM / by Simonetta Tumbiolo posted in SLE Supported Liquid Extraction, SPE, Extraction method optimization

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      Confused about how to choose the right sample preparation technique for your compound? Don't know if Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE) would be best for your sample preparation? We are here to help you select the most efficient extraction method for your sample, step by step, in the easiest possible way.

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