In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass. We have developed an adequate solid phase extraction method for our laboratory. However, we're starting to notice some oddities when we extract and analyze our samples. Could we be losing compounds from our sample?
Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interaction during SPE is useful when a large number of analytes with different properties are of interest.