The hydrolysis for both the EXPRESS CX and HYDRO CX methods was done using an incubation of 55C for 30 minutes.
Recoveries vary depending on the enzyme. However, they are consistent between the 2 plates.
As can be seen above, hydrolysis efficiencies, recoveries, and matrix effects are nearly identical for both the in-well hydrolysis plate and the out-of-well hydrolysis plate. For my future urine panels that require hydrolysis, I think I will be using the EVOLUTE HYDRO CX plates to save me a bit of time and to eliminate some cross-contamination risk.
Biotage published more information about the methods and comparisons between the two plates in a poster presented at ASMS 2017.
To read more about how the HYDRO plates work, please read Biotage EVOLUTE HYDRO CX application note.