Welcome to the Biotage Sample Preparation Blog

      Dilute & shoot ... yourself in the foot

      October 21, 2020 at 8:30 AM / by Simonetta Tumbiolo posted in SLE Supported Liquid Extraction, Sample preparation, Method development, Clinical

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      Urine samples are composed of many interferents which can cause ion-suppression or ion-enhancement when analyzing by mass spec. Build up of these matrix components over multiple sample injections can lead to loss of signal in the mass spec and even unexpected changes in your chromatographic separation. We are going to discuss why not performing adequate sample prep can be detrimental for your analyses.

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      How to achieve faster sample prep, cleaner extracts, and instant sensitivity gains

      October 15, 2020 at 9:06 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to meet goals for detection limits. What's best between conventional solid phase extraction (SPE) and microelution SPE?

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      The revolution of microelution in solid phase extraction

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be an effective tool when bioanalysis demands higher sensitivity and reproducibility with decreasing sample volumes? In theory, SPE can be used for targeted extractions with ultra-high concentrations. As we will see, putting this into practice requires miniaturization of SPE extraction using sorbent beds with optimal formats that minimize elution volume to maximize detection and quantitation power.

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      How to meet demands for detection limits with the right sample prep

      October 15, 2020 at 9:04 AM / by Nigel Tooke posted in Sample preparation, Solid-phase extraction, detection limit, Microelution

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      Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volumes. Achieving this performance involves pushing the limits of detection and quantitation with high levels of sensitivity and reproducibility that are only possible with high quality samples.

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      When should I choose SPE instead of SLE?

      October 9, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, SPE, Solid-phase extraction

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      When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques. 

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      Protein precipitation vs. traditional protein crash: what's best?

      October 7, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in Sample preparation, Method development, Protein precipitation

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      Most clinical chemists have developed a blood, serum or plasma assay using a protein crash because it is inexpensive and generally removes proteins that interfere with detection or the analysis in some way. But is this always true?

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      How do I Develop a Sample Prep Method?

      October 2, 2020 at 8:00 AM / by Stephanie J. Marin, Ph.D. posted in LC-MS, Sample preparation

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      This is a question that I am often asked by very smart people that just haven’t had time to learn the process. An optimized sample preparation method is critical for an accurate, specific, robust clinical LC-MS/MS assay. A good sample prep method can improve accuracy and precision, provide longer LC column lifetime, and keep your LC-MS/MS system clean.

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