Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.
In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass. We have developed an adequate solid phase extraction method for our laboratory. However, we're starting to notice some oddities when we extract and analyze our samples. Could we be losing compounds from our sample?
LC/MS/MS seems to be the gold standard in sample analysis nowadays. However, some labs are still analyzing samples using GC/MS. The majority of published peer-reviewed articles are for LC/MS/MS, so those working with GC/MS instrumentation may not know how to change the sample preparation to better fit GC/MS.
When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, the last thing we want to see is some random hiccup in our workflow. But a whole validation without a hiccup is just wishful thinking, right.
There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple guidelines on making the best choice. You will see that ion exchange is all about acids and bases.
When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s right for you depends on your application and the characteristics of your analytes. This post outlines some of the advantages, disadvantages and limitations of each technique.
What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SPE phases - EVOLUTE EXPRESS WCX and EVOLUTE EXPRESS WAX - and how to optimize your sample preparation of your analytes of interest.
