Welcome to the Biotage Sample Preparation Blog

      Vacuum or positive pressure for processing samples: what's best?

      July 29, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in SPE, Extraction method optimization, Sample preparation, Solid-phase extraction

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      Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of the benefits that we see when using positive pressure for SPE.

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      Determining the Optimal Sample Size for your Sorbent Bed Mass

      July 26, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in Extraction method optimization, Sample preparation, Evolute Express CX, Sorbent, Method development

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      In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass.  We have developed an adequate solid phase extraction method for our laboratory. However, we're starting to notice some oddities when we extract and analyze our samples. Could we be losing compounds from our sample?

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      How does my sample prep change between GC/MS and LC/MS/MS?

      July 23, 2020 at 8:00 AM / by Jillian Neifeld, MS posted in LC-MS, GC-MS, Sample preparation

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      LC/MS/MS seems to be the gold standard in sample analysis nowadays. However, some labs are still analyzing samples using GC/MS. The majority of published peer-reviewed articles are for LC/MS/MS, so those working with GC/MS instrumentation may not know how to change the sample preparation to better fit GC/MS.

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      How to Monitor and Prevent Sample Carryover during Method Development

      July 19, 2020 at 8:00 AM / by Dan Menasco posted in Troubleshooting and Optimization, Extraction method optimization, Sample preparation

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      When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, the last thing we want to see is some random hiccup in our workflow. But a whole validation without a hiccup is just wishful thinking, right.

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      Choosing the best Ion Exchange Mode for Solid Phase Extraction

      July 16, 2020 at 8:00 AM / by Bruce Kempf posted in SPE, Solid-phase extraction, ION exchange, Evolute Express

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      There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple guidelines on making the best choice. You will see that ion exchange is all about acids and bases.

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      When should I choose SLE or SPE for sample cleanup?

      July 13, 2020 at 9:21 AM / by Bruce Kempf posted in SLE Supported Liquid Extraction, SPE

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      When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s right for you depends on your application and the characteristics of your analytes. This post outlines some of the advantages, disadvantages and limitations of each technique.

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      When should I choose weak ion exchange SPE phases?

      July 8, 2020 at 3:17 PM / by Stephanie J. Marin, Ph.D. posted in SPE, Extraction method optimization, Sample preparation

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      What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SPE phases - EVOLUTE EXPRESS WCX and EVOLUTE EXPRESS WAX - and how to optimize your sample preparation of your analytes of interest.

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      When should I choose a mixed-mode SPE?

      July 8, 2020 at 3:15 PM / by Stephanie J. Marin, Ph.D. posted in SPE, Evolute Express AX, Evolute Express CX

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      Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interaction during SPE is useful when a large number of analytes with different properties are of interest.

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