Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.
Developing a new sample prep method should be systematic to ensure ruggedness, and can be done quickly with the right tools. The days of using a vacuum manifold are gone, along with the variability and long extraction times incurred. Today’s options provide an array of positive pressure systems that process a wide range of sample matrices, varying in viscosities.
So you’ve decided that you need to develop some new methods and one of the biggest challenges is the cost of developing and validating new methods. So, what to do to speed up the process?
Many Labs today are faced with the challenge of having to do more with existing personnel and space. When looking at developing or modifying a LC/MS or GC/MS assay, one of the most important steps is the sample prep.
Microelution SPE is a great alternative to conventional SPE methods, enabling the use of lower wash and elution volumes, while maintaining sufficient load volumes, providing concentrated samples, perfect for highly sensitive analysis. Achieving the best, most consistent results means using the right processing conditions, and because all microelution SPE plates are not the same, you might need to adjust your processing conditions when using these plates.
A new method has been developed for the combined analysis of Vitamin A, Vitamin D, Vitamin E and Vitamin K from serum using LC-MS/MS. The method uses supported liquid extraction (SLE) with a novel sample load which could also potentially be employed for many other highly non-polar analytes that have limited water solubility and are highly protein bound within the body.
What's the process behind tuning our analytes to the mass spectrometer’s optics? And why is that so important?