Welcome to the Biotage Sample Preparation Blog

      How to choose the best elution solvent for SLE?

      September 24, 2021 at 2:18 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, solvent polarity, elution

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      Choosing the SLE correct elution solvent based on analyte properties produces the cleanest extracts with the highest recoveries.

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      How do I pretreat my samples for Supported Liquid Extraction

      July 14, 2021 at 6:09 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction, pretreatment

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      Correct pretreatment of samples prior to supported liquid extraction (SLE) produces better partitioning and cleaner extracts. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      Why should I underload SLE+ plate or cartridge?

      July 7, 2021 at 7:00 PM / by Stephanie J. Marin, Ph.D. posted in SLE Supported Liquid Extraction, Sample preparation, extraction

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      Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on a solid supported surface.

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      Setting yourself up for Success

      May 27, 2021 at 8:36 AM / by Jeremy Smith posted in LC-MS, GC-MS, SPE, Positive Pressure, High Throughput, Low Volume Extractions, mass spec

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      Developing a new sample prep method should be systematic to ensure ruggedness, and can be done quickly with the right tools. The days of using a vacuum manifold are gone, along with the variability and long extraction times incurred. Today’s options provide an array of positive pressure systems that process a wide range of sample matrices, varying in viscosities.

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      Challenges in developing new laboratory methods...

      May 27, 2021 at 8:05 AM / by Jillian Neifeld, MS posted in LC-MS, GC-MS, SPE, High Throughput, Low Volume Extractions, mass spec

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      So you’ve decided that you need to develop some new methods and one of the biggest challenges is the cost of developing and validating new methods. So, what to do to speed up the process?

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      No one wants to do sample prep if they don’t have to...

      May 27, 2021 at 8:00 AM / by Elena Gairloch posted in LC-MS, GC-MS, SPE, High Throughput, Low Volume Extractions

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      Many Labs today are faced with the challenge of having to do more with existing personnel and space. When looking at developing or modifying a LC/MS or GC/MS assay, one of the most important steps is the sample prep.

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      Microelution plate processing: What pressure is best?

      March 16, 2021 at 3:33 PM / by Charlotte Hayes posted in Troubleshooting and Optimization, Extraction method optimization, Microelution, SPE, positive pressure processing

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      Microelution SPE is a great alternative to conventional SPE methods, enabling the use of lower wash and elution volumes, while maintaining sufficient load volumes, providing concentrated samples, perfect for highly sensitive analysis. Achieving the best, most consistent results means using the right processing conditions, and because all microelution SPE plates are not the same, you might need to adjust your processing conditions when using these plates.

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      Why just measure Vitamin D when you can monitor Vitamins A, E and K as well?

      November 11, 2020 at 2:58 PM / by Alan Edgington posted in SLE Supported Liquid Extraction, Sample preparation, Vitamins

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      A new method has been developed for the combined analysis of Vitamin A, Vitamin D, Vitamin E and Vitamin K from serum using LC-MS/MS. The method uses supported liquid extraction (SLE) with a novel sample load which could also potentially be employed for many other highly non-polar analytes that have limited water solubility and are highly protein bound within the body.

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      Troubleshooting Loss of Signal: Where did my peaks go?

      October 25, 2020 at 7:45 AM / by Dan Menasco posted in Troubleshooting and Optimization, Sample preparation

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      Let’s take a look at one of the more irritating issues in LC/MS method development: signal loss. I’m not talking about signal attenuation, which can be much more convoluted to diagnose; but rather a complete loss of signal – nothing, not even a blip on your TIC. So where do we begin?

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      Putting it Together: why should I tune my mass spectrometer?

      October 25, 2020 at 7:30 AM / by Dan Menasco posted in Chromatography Fundamentals, Sample preparation

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      What's the process behind tuning our analytes to the mass spectrometer’s optics? And why is that so important?

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